Sex zup

Brains were removed from two male and two female pups on PN0 within 6 h of birth and placed in a Zivic Miller brain mold.A 3-mm section was cut that contained both the POA and medial basal hypothalamus (MBH) (13).On the day of birth (PN0), phosphorylation of the c AMP response element-binding protein (CREB) is elevated in the ventromedial nucleus of the hypothalamus and the POA of the male.Females administered estradiol on PN0 have increased phosphorylated CREB (p CREB) in these areas on PN1 relative to vehicle-treated controls (7).Adult Sprague Dawley rats (Charles River Laboratories, Wilmington, MA) were bred in our animal facility.Pregnancy was confirmed by the presence of sperm in the vaginal lavage.

Once it reaches the brain, testosterone is converted to estradiol by the aromatase enzyme.females during the critical period for sexual differentiation of the brain.To identify other signaling molecules differentially regulated in the male and female hypothalamus during the hormonally sensitive critical period, we used a high-throughput proteomics approach called Power Blot (BD Biosciences Phar Mingen, La Jolla, CA).There are sex differences in signal transduction in the neonatal rat brain.Expression of the immediate early gene, Fos, is elevated in the medial preoptic area (m POA) of the male compared with the female during the first several days of life (6).

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